A Fluorometric CYP19A1 (Aromatase) Activity Assay in Live Cells.
| Author | |
|---|---|
| Abstract |
:
Inhibition of estrogen synthesis is an integral component of the frontline pharmacologic therapy for the treatment of estrogen receptor positive cancers. However, there is currently no direct, high-throughput-ready assay for aromatase (also known as CYP19A1) that can be performed in live cells. Herein we present a cell-based assay that allows for multiplexed assessment of enzyme activity, protein half-life, cell viability, and identification of inhibitors with slow off-rates. |
| Year of Publication |
:
2021
|
| Journal |
:
ChemMedChem
|
| Volume |
:
16
|
| Issue |
:
18
|
| Number of Pages |
:
2845-2850
|
| Date Published |
:
2021
|
| ISSN Number |
:
1860-7179
|
| URL |
:
https://doi.org/10.1002/cmdc.202100326
|
| DOI |
:
10.1002/cmdc.202100326
|
| Short Title |
:
ChemMedChem
|
| Download citation |
